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Öztürk, Merve; Freiwald, Anja; Cartano, Jasmin; Schmitt, Ramona; Dejung, Mario; Luck, Katja; Al-Sady, Bassem; Braun, Sigurd; Levin, Michal; Butter, Falk (, Nature Communications)Abstract Protein abundance is controlled at the transcriptional, translational and post-translational levels, and its regulatory principles are starting to emerge. Investigating these principles requires large-scale proteomics data and cannot just be done with transcriptional outcomes that are commonly used as a proxy for protein abundance. Here, we determine proteome changes resulting from the individual knockout of 3308 nonessential genes in the yeast Schizosaccharomyces pombe . We use similarity clustering of global proteome changes to infer gene functionality that can be extended to other species, such as humans or baker’s yeast. Furthermore, we analyze a selected set of deletion mutants by paired transcriptome and proteome measurements and show that upregulation of proteins under stable transcript expression utilizes optimal codons.more » « less
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Levin, Michal; Valentine, Megan T.; Cohen, Noy (, Journal of the Mechanical Behavior of Biomedical Materials)null (Ed.)
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Levin, Michal; Tang, Yongkui; Eisenbach, Claus_D; Valentine, Megan_T; Cohen, Noy (, Macromolecules)
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